As such, mass photometry could be ideally suited esatto address the shortcomings of existing fluorescence-based techniques for durante vitro applications esatto studying IMPs and MAPs
State-of-the-art single-molecule approaches rely largely on the addition of fluorescent labels, which complicates the quantification of the involved stoichiometries and dynamics because of low temporal resolution and the inherent limitations associated with labeling efficiency, photoblinking and photobleaching. Here, we demonstrate dynamic mass photometry, verso method for label-free imaging, tracking and mass measurement of individual membrane-associated proteins diffusing on supported lipid bilayers. Application of this method puro the membrane remodeling GTPase, dynamin-1, reveals heterogeneous mixtures of dimer-based oligomers, oligomer-dependent mobilities, membrane affinities and (dis)association of individual complexes. These capabilities, together with assay-based advances for studying integral membrane proteins, will enable the elucidation of biomolecular mechanisms per and on lipid bilayers.
The quantification of membrane-associated biomolecular interactions is crucial sicuro our understanding of various cellular processes
Integral membrane proteins (IMPs) and membrane-associated proteins (MAPs) are essential for per number of cellular processes such as signaling and vesicular trafficking, and this makes them important therapeutic targets 1,2 . More…
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